Fatty Acids and Metabolomic Composition of Follicular Fluid Collected from Environments Associated with Good and Poor Oocyte Competence in Goats
Izquierdo Tugas, Ma. Dolors (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Roura, Montserrat (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Pérez-Trujillo, Míriam (Universitat Autònoma de Barcelona. Servei de Ressonància Magnètica Nuclear)
Soto-Heras, Sandra (University of Illinois Urbana-Champaign. Department of Animal Sciences)
Paramio, María Teresa (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)

Data:	2022
Resum:	In goats, embryo oocyte competence is affected by follicle size, regardless of the age of the females. In previous studies, we found differences in blastocyst development between oocytes coming of small (< 3 mm) and large follicles (> 3 mm) in prepubertal (1-2 month-old) goats. Oocyte competence and follicular fluid (FF) composition changes throughout follicle growth. The aim of this study was to analyze the fatty acids (FAs) composition and metabolomic profiles of FF recovered from small and large follicles of prepubertal goats and follicles of adult goats. FAs were analyzed by chromatography and metabolites by 1H-nuclear magnetic resonance (1H-NMR) spectrometry. The results showed important differences between adult and prepubertal follicles: a) the presence of α,β-glucose in adult and no detection in prepubertal; b) lactate, -N-(CH3)3 groups and inositol were higher in prepubertal; c) the percentage of linolenic acid, total saturated fatty acids and n-3 PUFAs were higher in adults; and d) the percentage of linoleic acid, total MUFAs, PUFAs, n-6 PUFAs and n-6 PUFAs:n-3 PUFAs ratio were higher in prepubertal goats. No significant differences were found in the follicle size of prepubertal goats, despite the differences in oocyte competence for in vitro embryo production.
Ajuts:	Ministerio de Ciencia e Innovación AGL2017-85837-R
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Reproductive fluids ; Fertilization ; Follicles ; Omega-3 ; Omega-6 ; Glucose
Publicat a:	International journal of molecular sciences, Vol. 23 Núm. 8 (4-2 2022) , p. 4141, ISSN 1422-0067

DOI: 10.3390/ijms23084141
PMID: 35456957

15 p, 1.8 MB

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