Detection and quantification of the hypersensitive response cell death in Arabidopsis thaliana
Salguero Linares, José (Centre de Recerca en Agrigenòmica)
Lema A., Saul (Universidad de las Américas (Ecuador))
Salas Gómez, Marta (Centre de Recerca en Agrigenòmica)
Froilán Soares, Andrea (Centre de Recerca en Agrigenòmica)
Sánchez Coll, Núria (Centre de Recerca en Agrigenòmica)

Imprint:	New York : Humana, 2022
Description:	12 pàg.
Abstract:	In plants, the hypersensitive response (HR) is a programmed cell death modality that occurs upon recognition of harmful non-self. It occurs at the site of pathogen infection, thus preventing pathogens to live off plant tissue and proliferate. Shedding light on the molecular constituents underlying this process requires robust and quantitative methods that can determine whether plants lacking functional genes are defective in HR execution compared to wild-type controls. In this chapter, we provide two quantitative protocols in which we measure cell death from Arabidopsis thaliana leaves infected with avirulent HR-causing bacterial strains. Firstly, we use trypan blue staining to quantify the stained area of leaves upon bacterial infection using a personalized macro in the Image J (Fiji) software. Alternately, we incorporate an electrolyte leakage protocol in order to measure HR caused by different avirulent bacterial strains at different bacterial titers. We encourage users to perform a combination of both methods when assessing HR in different plant genotypes.
Grants:	Agencia Estatal de Investigación PID2019-108595RB-I00 Ministerio de Ciencia e Innovación AGL2016-78002-R Ministerio de Ciencia e Innovación BES-2017-080210 Ministerio de Economía y Competitividad SEV-2015-0533 Ministerio de Ciencia e Innovación CEX2019-000902-S
Note:	Altres ajuts: CERCA Programme/Generalitat de Catalunya
Rights:	Tots els drets reservats.
Language:	Anglès
Document:	Capítol de llibre ; Versió acceptada per publicar
Subject:	Arabidopsis thaliana ; Hypersensitive response ; Pseudomonas syringae pv tomato DC3000 ; Trypan Blue Staining ; Cell death quantification by Image J ; Electrolyte leakage
Published in:	Plant Proteases and Plant Cell Death: Methods and protocols, 2022, p. 193-204, ISBN 978-1-0716-2079-3

DOI: 10.1007/978-1-0716-2079-3_16

Available from: 2024-05-31 Postprint 19 p, 533.1 KB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Books and collections > Book chapters