Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine Embryos
Ordóñez-León, Erika Alina (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Martínez-Rodero, Iris (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Garcia Martinez, Tania (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
López Béjar, Manel (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Yeste Oliveras, Marc (Universitat de Girona. Departament de Biologia)
Mercadé, Elena (Universitat de Barcelona. Departament de Biologia)
Mogas Amorós, Teresa (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)

Data:	2022
Resum:	This study aimed to assess the cryoprotectant role of exopolysaccharide (EPS) ID1, produced by Antarctic Pseudomonas sp. , in the vitrification of in vitro-produced (IVP) bovine embryos. IVP day 7 (D7) and day 8 (D8) expanded blastocysts derived from cow or calf oocytes were vitrified without supplementation (EPS0) or supplemented with 10 µg/mL (EPS10) or 100 µg/mL (EPS100) EPS ID1. The effect of EPS ID1 was assessed in post-warming re-expansion and hatching rates, differential cell count, apoptosis rate, and gene expression. EPS100 re-expansion rates were significantly higher than those observed for the EPS0 and EPS10 treatments, regardless of culture length or oocyte source. EPS100 hatching rate was similar to the one of the fresh blastocysts except for those D7 blastocysts derived from calf oocytes. No differences were observed among EPS ID1 treatments when the inner cell mass, trophectoderm, and total cell number were assessed. Although apoptosis rates were higher (p ≤ 0. 05) in vitrified groups compared to fresh embryos, EPS100 blastocysts had a lower number (p ≤ 0. 05) of apoptotic nuclei than the EPS0 or EPS10 groups. No differences in the expression of BCL2, AQP3, CX43, and SOD1 genes between treatments were observed. Vitrification without EPS ID1 supplementation produced blastocysts with significantly higher BAX gene expression, whereas treatment with 100 µg/mL EPS ID1 returned BAX levels to those observed in non-vitrified blastocysts. Our results suggest that 100 µg/mL EPS ID1 added to the vitrification media is beneficial for embryo cryopreservation because it results in higher re-expansion and hatching ability and it positively modulates apoptosis.
Ajuts:	Ministerio de Ciencia e Innovación PID2020-116531RB-I00 Ministerio de Ciencia e Innovación BES-2017-081962
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; Versió publicada
Matèria:	Cryopreservation ; Blastocyst ; Total cell number ; Inner cell mass ; TUNEL ; Embryo development ; Gene expression regulation
Publicat a:	International journal of molecular sciences, Vol. 23 Núm. 13 (2022) , p. 7069, ISSN 1422-0067

DOI: 10.3390/ijms23137069
PMID: 35806071

18 p, 1.4 MB

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