Expression of Chimeric HPV-HIV Protein L1P18 in Pichia pastoris; Purification and Characterization of the Virus-like Particles
Eto, Yoshiki (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Saubi, Narcis (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Joseph-Munné, Joan (Hospital Universitari Vall d'Hebron)

Data:	2021
Resum:	Currently, three human papillomavirus (HPV) vaccines are already licensed and all of them are based on virus-like particles (VLPs) of HPV L1 capsid protein but not worldwide accessible. While about 38. 0 million people were living with HIV in 2019, only 68% of HIV-infected individuals were accessing antiretroviral therapy as of the end of June 2020 and there is no HIV vaccine yet. Therefore, safe, effective, and affordable vaccines against those two viruses are immediately needed. Both HPV and HIV are sexually transmitted infections and one of the main access routes is the mucosal genital tract. Thus, the development of a combined vaccine that would protect against HPV and HIV infections is a logical effort in the fight against these two major global pathogens. In this study, a recombinant Pichia pastoris producing chimeric HPV-HIV L1P18 protein intracellularly was constructed. After cell disruption, the supernatant was collected, and the VLPs were purified by a combination of ammonium sulfate precipitation, size exclusion chromatography, ultracentrifugation, and ultrafiltration. At the end of purification process, the chimeric VLPs were recovered with 96% purity and 9. 23% overall yield, and the morphology of VLPs were confirmed by transmission electron microscopy. This work contributes towards the development of an alternative platform for production of a bivalent vaccine against HPV and HIV in P. pastoris.
Ajuts:	European Commission 681137 Instituto de Salud Carlos III RD12/0017 Instituto de Salud Carlos III PI14/00494 Instituto de Salud Carlos III PI20/00217
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	HPV ; HIV ; Virus-like particle ; Chimeric ; Vaccine ; Yeast ; Pichia pastoris ; Purification
Publicat a:	Pharmaceutics, Vol. 13, Issue 11 (November 2021) , art. 1967, ISSN 1999-4923

DOI: 10.3390/pharmaceutics13111967
PMID: 34834382

17 p, 14.9 MB

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