Detection and elimination of cellular bottlenecks in protein-producing yeasts
Zahrl, Richard J. (University of Natural Resources and Life Sciences. Department of Biotechnology (Austria))
Gasser, Brigitte (University of Natural Resources and Life Sciences. Department of Biotechnology (Austria))
Mattanovich, Diethard (University of Natural Resources and Life Sciences. Department of Biotechnology (Austria))
Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)

Imprint:	New York : Humana, 2019
Abstract:	Yeasts are efficient cell factories and are commonly used for the production of recombinant proteins for biopharmaceutical and industrial purposes. For such products high levels of correctly folded proteins are needed, which sometimes requires improvement and engineering of the expression system. The article summarizes major breakthroughs that led to the efficient use of yeasts as production platforms and reviews bottlenecks occurring during protein production. Special focus is given to the metabolic impact of protein production. Furthermore, strategies that were shown to enhance secretion of recombinant proteins in different yeast species are presented.
Grants:	Ministerio de Economía, Industria y Competitividad CTQ2016-74959-R Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-1462
Rights:	Tots els drets reservats.
Language:	Anglès
Series:	Methods in Molecular Biology ; 1923
Document:	Capítol de llibre ; Versió acceptada per publicar
Subject:	Yeasts ; Protein production ; Secretion ; Chaperones ; Protein degradation ; Metabolism ; Promoters
Published in:	Recombinant Protein Production in Yeast, 2019, p. 75-95, ISBN 978-1-4939-9024-5

DOI: 10.1007/978-1-4939-9024-5_2
PMID: 30737735

Postprint 24 p, 556.9 KB

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