Improvement of the Polyhydroxyalkanoates Recovery from Mixed Microbial Cultures Using Sodium Hypochlorite Pre-Treatment Coupled with Solvent Extraction
Montiel-Jarillo, Gabriela (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Morales-Urrea, Diego A. (Instituto de Investigaciones en Ciencia y Tecnología de Materiales)
Contreras, Edgardo (Instituto de Investigaciones en Ciencia y Tecnología de Materiales)
López-Córdoba, Alex (Universidad Pedagógica y Tecnológica de Colombia. Escuela de Administración de Empresas Agropecuarias)
Gómez-Pachón, Edwin Yesid (Universidad Pedagógica y Tecnológica de Colombia. Escuela de Diseño Industrial)
Carrera, Julian (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Suárez Ojeda, María Eugenia (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)

Fecha:	2022
Resumen:	The use of mixed microbial cultures (MMC) and organic wastes and wastewaters as feed sources is considered an appealing approach to reduce the current polyhydroxyalkanoates (PHAs) production costs. However, this method entails an additional hurdle to the PHAs downstream processing (recovery and purification). In the current work, the effect of a sodium hypochlorite (NaClO) pre-treatment coupled with dimethyl carbonate (DMC) or chloroform (CF) as extraction solvents on the PHAs recovery efficiency (RE) from MMC was evaluated. MMC were harvested from a sequencing batch reactor (SBR) fed with a synthetic prefermented olive mill wastewaster. Two different carbon-sources (acetic acid and acetic/propionic acids) were employed during the batch accumulation of polyhydroxybutyrate (PHB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) from MMC. Obtained PHAs were characterized by 1 H and 13 C nuclear magnetic resonance, gel-permeation chromatography, differential scanning calorimetry, and thermal gravimetric analysis. The results showed that when a NaClO pre-treatment is not added, the use of DMC allows to obtain higher RE of both biopolymers (PHB and PHBV), in comparison with CF. In contrast, the use of CF as extraction solvent required a pre-treatment step to improve the PHB and PHBV recovery. In all cases, RE values were higher for PHBV than for PHB.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Polyhydroxyalkanoates ; Mixed microbial cultures ; Extraction ; Sodium hypochlorite ; Dimethyl carbonate ; Chloroform
Publicado en:	Polymers, Vol. 14, Issue 19 (September 2022) , art. 3938, ISSN 2073-4360

DOI: 10.3390/polym14193938
PMID: 36235886

17 p, 3.9 MB

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