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Página principal > Artículos > Artículos publicados > Antibody cooperative adsorption onto AuNPs and its exploitation to force natural killer cells to kill HIV-infected T cells |
Fecha: | 2021 |
Resumen: | HIV represents a persistent infection which negatively alters the immune system. New tools to reinvigorate different immune cell populations to impact HIV are needed. Herein, a novel nanotool for the specific enhancement of the natural killer (NK) immune response towards HIV-infected T-cells has been developed. Bispecific Au nanoparticles (BiAb-AuNPs), dually conjugated with IgG anti-HIVgp120 and IgG anti-human CD16 antibodies, were generated by a new controlled, linker-free and cooperative conjugation method promoting the ordered distribution and segregation of antibodies in domains. The cooperatively-adsorbed antibodies fully retained the capabilities to recognize their cognate antigen and were able to significantly enhance cell-to-cell contact between HIV-expressing cells and NK cells. As a consequence, the BiAb-AuNPs triggered a potent cytotoxic response against HIV-infected cells in blood and human tonsil explants. Remarkably, the BiAb-AuNPs were able to significantly reduce latent HIV infection after viral reactivation in a primary cell model of HIV latency. This novel molecularly-targeted strategy using a bispecific nanotool to enhance the immune system represents a new approximation with potential applications beyond HIV. |
Ayudas: | Ministerio de Economía y Competitividad RTI2018-101082-B-I00 Ministerio de Economía y Competitividad SAF2015-67334-R Instituto de Salud Carlos III PI17/01470 Instituto de Salud Carlos III PI14/01058 Instituto de Salud Carlos III PI14/00179 Instituto de Salud Carlos III CPII15-000014 Ministerio de Ciencia e Innovación BES-2016-076382 Agència de Gestió d'Ajuts Universitaris i de Recerca FI-B-00582 |
Derechos: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades. |
Lengua: | Anglès |
Documento: | Article ; recerca ; Versió publicada |
Materia: | HIV ; Bispecific nanoparticles ; NK cells ; Polarization |
Publicado en: | Nano Today, Vol. 36 (Feb. 2021) , art. 101056, ISSN 1878-044X |
15 p, 4.8 MB |