Effect of cryoprotectant concentration on bovine oocyte permeability and comparison of two membrane permeability modelling approaches
Garcia Martinez, Tania (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Mogas Amorós, Teresa (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Mullen, Steven F. (COOK Medical)
Martínez-Rodero, Iris (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Gulieva, Ramila E. (Oregon State University. School of Chemical, Biological and Environmental Engineering)
Higgins, Adam Z. (Oregon State University. School of Chemical, Biological and Environmental Engineering)

Date:	2021
Abstract:	The plasma membrane permeability to water and cryoprotectant (CPA) significantly impacts vitrification efficiency of bovine oocytes. Our study was designed to determine the concentration-dependent permeability characteristics for immature (GV) and mature (MII) bovine oocytes in the presence of ethylene glycol (EG) and dimethyl sulphoxide (MeSO), and to compare two different modeling approaches: the two parameter (2P) model and a nondilute transport model. Membrane permeability parameters were determined by consecutively exposing oocytes to increasing concentrations of MeSO or EG. Higher water permeability was observed for MII oocytes than GV oocytes in the presence of both MeSO and EG, and in all cases the water permeability was observed to decrease as CPA concentration increased. At high CPA concentrations, the CPA permeability was similar for MeSO and EG, for both MII and GV oocytes, but at low concentrations the EG permeability of GV oocytes was substantially higher. Predictions of cell volume changes during CPA addition and removal indicate that accounting for the concentration dependence of permeability only has a modest effect, but there were substantial differences between the 2P model and the nondilute model during CPA removal, which may have implications for design of improved methods for bovine oocyte vitrification.
Grants:	Ministerio de Ciencia e Innovación Project AGL2016-79802-P
Note:	Generalitat de Catalunya 2019 FI_B2 00055
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Biophysics ; Cell biology ; Developmental biology ; Molecular biology
Published in:	Scientific reports, Vol. 11 (july 2021) , ISSN 2045-2322

DOI: 10.1038/s41598-021-94884-0
PMID: 34321576

15 p, 1.7 MB

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