Priming human adipose-derived mesenchymal stem cells for corneal surface regeneration
Nieto-Nicolau, Núria (Universitat de Barcelona. Departament de Biologia Cel·lular)
Martínez-Conesa, Eva M (Institut d'Investigació Biomèdica Sant Pau)
Fuentes-Julián, S. (Instituto de Investigación Sanitaria del Hospital Universitario La Paz)
Arnalich-Montiel, Francisco (Instituto de Investigación Sanitaria del Hospital Universitario La Paz)
García-Tuñón, I. (Instituto de Investigación Sanitaria del Hospital Universitario La Paz)
De Miguel, M.P. (Instituto de Investigación Sanitaria del Hospital Universitario La Paz)
Casaroli-Marano, Ricardo Pedro (Institut d'Investigació Biomèdica Sant Pau)

Date:	2021
Abstract:	Limbal stem cells (LSC) maintain the transparency of the corneal epithelium. Chemical burns lead the loss of LSC inducing an up-regulation of pro-inflammatory and pro-angiogenic factors, triggering corneal neovascularization and blindness. Adipose tissue-derived mesenchymal stem cells (AT-MSC) have shown promise in animal models to treat LSC deficiency (LSCD), but there are not studies showing their efficacy when primed with different media before transplantation. We cultured AT-MSC with standard medium and media used to culture LSC for clinical application. We demonstrated that different media changed the AT-MSC paracrine secretion showing different paracrine effector functions in an in vivo model of chemical burn and in response to a novel in vitro model of corneal inflammation by alkali induction. Treatment of LSCD with AT-MSC changed the angiogenic and inflammatory cytokine profile of mice corneas. AT-MSC cultured with the medium that improved their cytokine secretion, enhanced the anti-angiogenic and anti-inflammatory profile of the treated corneas. Those corneas also presented better outcome in terms of corneal transparency, neovascularization and histologic reconstruction. Priming human AT-MSC with LSC specific medium can potentiate their ability to improve corneal wound healing, decrease neovascularization and inflammation modulating paracrine effector functions in an in vivo optimized rat model of LSCD.
Grants:	Instituto de Salud Carlos III FIS14-PI00196 Instituto de Salud Carlos III FIS18-PI00355 Instituto de Salud Carlos III PI15-01447 Ministerio de Educación, Cultura y Deporte AP2010-0659
Note:	Altres ajuts: Fundació la Marató de TV 20120630-30-31
Note:	Altres ajuts: Fondo Europeo de Desarrollo Regional (FEDER)
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Cell culture priming ; Cytokines ; Directed differentiation media ; Limbal stem cells ; Limbal stem cells deficiency ; Paracrine secretion
Published in:	Journal of Cellular and Molecular Medicine, Vol. 25 Núm. 11 (june 2021) , p. 5124-5137, ISSN 1582-4934

DOI: 10.1111/jcmm.16501
PMID: 33951289

14 p, 2.3 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Recerca Sant Pau
Articles > Research articles
Articles > Published articles