Flow-cytometry-based protocols for human blood/marrow immunophenotyping with minimal sample perturbation
G. Rico, Laura (Institut Germans Trias i Pujol. Institut de Recerca contra la Leucèmia Josep Carreras)
Salvia, Roser (Institut Germans Trias i Pujol. Institut de Recerca contra la Leucèmia Josep Carreras)
Ward, Michael D. (Thermo Fisher Scientific)
Bradford, Jolene A. (Thermo Fisher Scientific)
Petriz, Jordi (Institut Germans Trias i Pujol. Institut de Recerca contra la Leucèmia Josep Carreras)

Date:	2021
Abstract:	This protocol provides instructions to improve flow cytometry analysis of marrow/peripheral blood cells by avoiding erythrolytic solutions, density gradients, and washing steps. We describe two basic approaches for identifying cell surface antigens with minimal sample perturbation, which have been successfully used to identify healthy and pathologically rare cells. The greatest advantage of these approaches is that they minimize the unwanted effect caused by sample preparation, allowing for improved study of live cells at the point of analysis. For complete details on the use and execution of this protocol, please refer to Petriz et al. (2018).
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Altres ; recerca ; Versió publicada
Subject:	Flow cytometry/Mass cytometry ; Cancer ; Health sciences ; Clinical protocol ; Immunology ; Stem Cells
Published in:	STAR Protocols, Vol. 2 Núm. 4 (17 2021) , art. 100883, ISSN 2666-1667

DOI: 10.1016/j.xpro.2021.100883
PMID: 34693361

20 p, 7.0 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP) > Josep Carreras Leukaemia Research Institute
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Articles > Published articles