The Footprint of Type 1 Diabetes on Red Blood Cells : A Metabolomic and Lipidomic Study
Herance, José Raul (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Ciudin, Andreea (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Lamas-Domingo, Rubén (Centro de Investigación Príncipe Felipe (València))
Aparicio-Gómez, Carolina (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Hernández, Cristina (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Simó Canonge, Rafael (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Palomino-Schätzlein, Martina (Parque Tecnológico de Valencia)
Universitat Autònoma de Barcelona

Data:	2023
Resum:	The prevalence of diabetes type 1 (T1D) in the world populations is continuously growing. Although treatment methods are improving, the diagnostic is still symptom-based and sometimes far after onset of the disease. In this context, the aim of the study was the search of new biomarkers of the disease in red blood cells (RBCs), until now unexplored. The metabolomic and the lipidomic profile of RBCs from T1D patients and matched healthy controls was determined by NMR spectroscopy, and different multivariate discrimination models were built to select the metabolites and lipids that change most significantly. Relevant metabolites were further confirmed by univariate statistical analysis. Robust separation in the metabolomic and lipidomic profiles of RBCs from patients and controls was confirmed by orthogonal projection on latent structure discriminant analysis (OPLS-DA), random forest analysis, and significance analysis of metabolites (SAM). The main changes were detected in the levels of amino acids, organic acids, creatine and phosphocreatine, lipid change length, and choline derivatives, demonstrating changes in glycolysis, BCAA metabolism, and phospholipid metabolism. Our study proves that robust differences exist in the metabolic and lipidomic profile of RBCs from T1D patients, in comparison with matched healthy individuals. Some changes were similar to alterations found already in RBCs of T2D patients, but others seemed to be specific for type 1 diabetes. Thus, many of the metabolic differences found could be biomarker candidates for an earlier diagnosis or monitoring of patients with T1D.
Ajuts:	Ministerio de Economía y Competitividad PI16/02064 Instituto de Salud Carlos III PI20/01588 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017SGR1303
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Type 1 diabetes ; NMR spectroscopy ; Metabolomics ; Lipidomics ; Red blood cells
Publicat a:	Journal of clinical medicine, Vol. 12 (january 2023) , ISSN 2077-0383

DOI: 10.3390/jcm12020556
PMID: 36675484

14 p, 2.1 MB

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