The Modular Architecture of Metallothioneins Facilitates Domain Rearrangements and Contributes to Their Evolvability in Metal-Accumulating Mollusks
Calatayud, Sara (Universitat de Barcelona. Departament de Genètica, Microbiologia i Estadística)
García-Risco, Mario (Universitat Autònoma de Barcelona. Departament de Química)
Pedrini-Martha, Veronika (University of Innsbruck. Department of Zoology)
Niederwanger, Michael (University of Innsbruck. Department of Zoology)
Dallinger, Reinhard (University of Innsbruck. Department of Zoology)
Palacios Bonilla, Òscar (Universitat Autònoma de Barcelona. Departament de Química)
Capdevila Vidal, Mercè (Universitat Autònoma de Barcelona. Departament de Química)
Albalat, Ricard (Universitat de Barcelona. Institut de Recerca de la Biodiversitat (IRBio))

Data:	2022
Resum:	Protein domains are independent structural and functional modules that can rearrange to create new proteins. While the evolution of multidomain proteins through the shuffling of different preexisting domains has been well documented, the evolution of domain repeat proteins and the origin of new domains are less understood. Metallothioneins (MTs) provide a good case study considering that they consist of metal-binding domain repeats, some of them with a likely de novo origin. In mollusks, for instance, most MTs are bidomain proteins that arose by lineage-specific rearrangements between six putative domains: α, β1, β2, β3, γ and δ. Some domains have been characterized in bivalves and gastropods, but nothing is known about the MTs and their domains of other Mollusca classes. To fill this gap, we investigated the metal-binding features of NpoMT1 of Nautilus pompilius (Cephalopoda class) and FcaMT1 of Falcidens caudatus (Caudofoveata class). Interestingly, whereas NpoMT1 consists of α and β1 domains and has a prototypical Cd preference, FcaMT1 has a singular preference for Zn ions and a distinct domain composition, including a new Caudofoveata-specific δ domain. Overall, our results suggest that the modular architecture of MTs has contributed to MT evolution during mollusk diversification, and exemplify how modularity increases MT evolvability.
Ajuts:	Ministerio de Economía y Competitividad BIO2015-67358-C2-2-P Ministerio de Ciencia e Innovación PID2021-123258NB-I00 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-1665 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-864
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	International journal of molecular sciences, Vol. 23, Issue 24 (December 2022) , art. 15824, ISSN 1422-0067

DOI: 10.3390/ijms232415824
PMID: 36555472

12 p, 1.5 MB

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