Biochemical responses, feeding and survival in the solitary bee Osmia bicornis following exposure to an insecticide and a fungicide alone and in combination
Martins, Cátia Ariana Henriques (Alma Mater Studiorum Università Di Bologna. Department of Agricultural and Food Sciences)
Caliani, Ilaria (University of Siena. Department of Physical, Earth and Environmental Sciences)
D'Agostino, Antonella (University of Naples Parthenope. Department of Management and Quantitative Studies)
Di Noi, Agata (University of Siena. Department of Life Sciences)
Casini, Silvia (University of Siena. Department of Physical, Earth and Environmental Sciences)
Parrilli, Martina (Alma Mater Studiorum Università Di Bologna. Department of Agricultural and Food Sciences)
Azpiazu, Celeste (Universidad Politécnica de Madrid)
Bosch, Jordi 1961- (Centre de Recerca Ecològica i d'Aplicacions Forestals)
Sgolastra, Fabio (Alma Mater Studiorum Università Di Bologna. Department of Agricultural and Food Sciences)

Data:	2022
Resum:	In agricultural ecosystems, bees are exposed to combinations of pesticides that may have been applied at different times. For example, bees visiting a flowering crop may be chronically exposed to low concentrations of systemic insecticides applied before bloom and then to a pulse of fungicide, considered safe for bees, applied during bloom. In this study, we simulate this scenario under laboratory conditions with females of the solitary bee, Osmia bicornis L. We studied the effects of chronic exposure to the neonicotinoid insecticide, Confidor® (imidacloprid) at a realistic concentration, and of a pulse (1 day) exposure of the fungicide Folicur® SE (tebuconazole) at field application rate. Syrup consumption, survival, and four biomarkers: acetylcholinesterase (AChE), carboxylesterase (CaE), glutathione S-transferase (GST), and alkaline phosphatase (ALP) were evaluated at two different time points. An integrated biological response (IBRv2) index was elaborated with the biomarker results. The fungicide pulse had no impact on survival but temporarily reduced syrup consumption and increased the IBRv2 index, indicating potential molecular alterations. The neonicotinoid significantly reduced syrup consumption, survival, and the neurological activity of the enzymes. The co-exposure neonicotinoid-fungicide did not increase toxicity at the tested concentrations. AChE proved to be an efficient biomarker for the detection of early effects for both the insecticide and the fungicide. Our results highlight the importance of assessing individual and sub-individual endpoints to better understand pesticide effects on bees.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Biomarkers ; IBRv2 index ; Imidacloprid ; Pesticides ; Sub-lethal effects ; Tebuconazole
Publicat a:	Environmental Science and Pollution Research International, Vol. 30 (November 2022) , p. 27636-27649, ISSN 1614-7499

DOI: 10.1007/s11356-022-24061-x
PMID: 36383317

14 p, 1.3 MB

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