The Lack of Alternative Oxidase 1a Restricts in vivo Respiratory Activity and Stress-Related Metabolism for Leaf Osmoprotection and Redox Balancing Under Sudden Acute Water and Salt Stress in Arabidopsis thaliana
Fernández Del-Saz, Néstor (Universidad de Concepción)
Iglesias Sánchez, Ariadna (Centre de Recerca en Agrigenòmica)
Alonso-Forn, David (Centro de Investigación y Tecnología Agroalimentaria de Aragón)
López Gómez, Miguel (Universidad de Granada. Departamento de Fisiología Vegetal)
Palma Martín, Francisco José (Universidad de Granada. Departamento de Fisiología Vegetal)
Clemente-Moreno, Maria Jose (Universitat de les Illes Balears. Departament de Biologia)
Fernie, Alisdair (Max-Planck-Institut für Molekulare Pflanzenphysiologie)
Ribas-Carbo, Miquel (Universitat de les Illes Balears. Departament de Biologia)
Florez-Sarasa, I. (Institut de Recerca i Tecnologia Agroalimentàries)

Data:	2022
Resum:	In plants salt and water stress result in an induction of respiration and accumulation of stress-related metabolites (SRMs) with osmoregulation and osmoprotection functions that benefit photosynthesis. The synthesis of SRMs may depend on an active respiratory metabolism, which can be restricted under stress by the inhibition of the cytochrome oxidase pathway (COP), thus causing an increase in the reduction level of the ubiquinone pool. However, the activity of the alternative oxidase pathway (AOP) is thought to prevent this from occurring while at the same time, dissipates excess of reducing power from the chloroplast and thereby improves photosynthetic performance. The present research is based on the hypothesis that the accumulation of SRMs under osmotic stress will be affected by changes in folial AOP activity. To test this, the oxygen isotope-fractionation technique was used to study the in vivo respiratory activities of COP and AOP in leaves of wild-type Arabidopsis thaliana plants and of aox1a mutants under sudden acute stress conditions induced by mannitol and salt treatments. Levels of leaf primary metabolites and transcripts of respiratory-related proteins were also determined in parallel to photosynthetic analyses. The lack of in vivo AOP response in the aox1a mutants coincided with a lower leaf relative water content and a decreased accumulation of crucial osmoregulators. Additionally, levels of oxidative stress-related metabolites and transcripts encoding alternative respiratory components were increased. Coordinated changes in metabolite levels, respiratory activities and photosynthetic performance highlight the contribution of the AOP in providing flexibility to carbon metabolism for the accumulation of SRMs.
Ajuts:	Ministerio de Economía y Competitividad CTM2014-53902-C2-1-P Ministerio de Ciencia e Innovación PRE2018-083610 Agencia Estatal de Investigación RYC2019-027244-I
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Water stress ; Salinity ; Alternative oxidase ; Oxygen-isotope fractionation ; Primary metabolism ; Photosynthesis ; Arabidopsis thaliana
Publicat a:	Frontiers in plant science, Vol. 13 (May 2022) , art. 833113, ISSN 1664-462X

DOI: 10.3389/fpls.2022.833113
PMID: 35656009

15 p, 8.0 MB

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