Circuit-guided population acclimation of a synthetic microbial consortium for improved biochemical production
Kang, Chae Won (Pohang University of Science and Technology. Department of Chemical Engineering)
Lim, Hyun Gyu (Pohang University of Science and Technology. Department of Chemical Engineering)
Won, Jaehyuk (Chung-Ang University. Department of Chemistry)
Cha, Sanghak (Pohang University of Science and Technology. Department of Chemical Engineering)
Shin, Giyoung (Pohang University of Science and Technology. School of Interdisciplinary Bioscience and Bioengineering)
Yang, Jae-Seong (Centre de Recerca en Agrigenòmica)
Sung, Jaeyoung (Chung-Ang University. Department of Chemistry)
Jung, Gyoo Yeol (Pohang University of Science and Technology. Department of Chemical Engineering)

Fecha:	2022
Resumen:	Microbial consortia have been considered potential platforms for bioprocessing applications. However, the complexity in process control owing to the use of multiple strains necessitates the use of an efficient population control strategy. Herein, we report circuit-guided synthetic acclimation as a strategy to improve biochemical production by a microbial consortium. We designed a consortium comprising alginate-utilizing Vibrio sp. dhg and 3-hydroxypropionic acid (3-HP)-producing Escherichia coli strains for the direct conversion of alginate to 3-HP. We introduced a genetic circuit, named "Population guider", in the E. coli strain, which degrades ampicillin only when 3-HP is produced. In the presence of ampicillin as a selection pressure, the consortium was successfully acclimated for increased 3-HP production by 4. 3-fold compared to that by a simple co-culturing consortium during a 48-h fermentation. We believe this concept is a useful strategy for the development of robust consortium-based bioprocesses.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Acclimatization ; Alginates ; Ampicillin ; Escherichia coli ; Microbial Consortia
Publicado en:	Nature communications, Vol. 13 (November 2022) , art. 6506, ISSN 2041-1723

DOI: 10.1038/s41467-022-34190-z
PMID: 36344561

9 p, 1.4 MB

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