Autochthonous and imported tegumentary leishmaniasis in Catalonia (Spain) : Aetiological evolution in the last four decades and usefulness of different typing approaches based on biochemical, molecular and proteomic markers
Fernández-Arévalo, Anna (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Ballart, Cristina (Barcelona Institute for Global Health (ISGlobal))
Muñoz-Basagoiti, Jordana (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Basarte, Leire (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Lobato, Gonzalo (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Arnau Vila, Albert (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Abras, Alba (Universitat de Girona. Departament de Biologia)
Tebar, Silvia (Universitat de Barcelona. Departament de Biologia, Sanitat i Medi Ambient)
Llovet, Teresa (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Lami, Patrick (National Reference Centre for Leishmaniasis. University Hospital Centre of Montpellier. University of Montpellier)
Pratlong, Francine (National Reference Centre for Leishmaniasis. University Hospital Centre of Montpellier. University of Montpellier)
Alsina, Mercè (Universitat de Barcelona. Departament de Medicina)
Roé Crespo, Esther (Institut d'Investigació Biomèdica Sant Pau)
Puig Sanz, Lluís (Institut d'Investigació Biomèdica Sant Pau)
Muñoz-Batet, Carmen (Institut d'Investigació Biomèdica Sant Pau)
Gállego Culleré, M. (Montserrat) (Barcelona Institute for Global Health (ISGlobal))
Universitat Autònoma de Barcelona

Date:	2022
Abstract:	Leishmaniasis is a transmissible disease caused by Leishmania protozoa. Spain is endemic for both visceral and cutaneous leishmaniasis, the autochthonous aetiological agent being Leishmania infantum. Around the world, the L. donovani complex is associated with visceral symptoms, while any species of the Leishmania or Viannia subgenera affecting human can produce tegumentary forms. In a context of growing numbers of imported cases, associated with globalisation, the aim of this study was to analyse the aetiological evolution of human tegumentary leishmaniasis in a region of Spain (Catalonia). Fifty-six Leishmania strains, isolated from 1981 to 2018, were analysed using MLEE, gene sequencing (hsp70, rpoIILS, fh and ITS2) and MALDI-TOF. The utility of these different analytical methods was compared. The results showed an increase in leishmaniasis over the two last decades, particularly imported cases, which represented 39% of all cases studied. Leishmania infantum, L. major, L. tropica, L. braziliensis, L. guyanensis and L. panamensis were identified. The combination of molecular and enzymatic methods allowed the identification of 29 different strain types (A to AC). Strain diversity was higher in L. (Viannia), whilst the different L. major types were relatable with geo-temporal data. Among the autochthonous cases, type C prevailed throughout the studied period (39%). Minor types generally appeared within a short time interval. While all the techniques provided identical identification at the species complex level, MALDI-TOF and rpoIILS or fh sequencing would be the most suitable identification tools for clinical practice, and the tandem hsp70-ITS2 could substitute MLEE in the epidemiological field.
Grants:	Ministerio de Ciencia, Innovación y Universidades RD12/0018/0010 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017SGR00924 Agencia Estatal de Investigación CEX2018-000806-S
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	Transboundary and Emerging Diseases, Vol. 69 Núm. 3 (may 2022) , p. 1404-1418, ISSN 1865-1682

DOI: 10.1111/tbed.14107

15 p, 6.5 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Recerca Sant Pau
Articles > Research articles
Articles > Published articles