A New Integrative and Mobilizable Element Is a Major Contributor to Tetracycline Resistance in Streptococcus dysgalactiae subsp. equisimilis
López de Egea, Guillem (Instituto de Salud Carlos III)
González-Díaz, Aida (Instituto de Salud Carlos III)
Guédon, Gérard (Université de Lorraine)
Lao, Julie (Université Paris-Saclay)
Berbel, Dàmaris (Instituto de Salud Carlos III)
Casabella Pernas, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Marimón, José María (Osakidetza Basque Health Service)
Cercenado, Emilia (Hospital General Universitario Gregorio Marañón)
Fernández-Delgado, Lucía (Hospital Universitari de Bellvitge)
Chiapello, Hélène (Université Paris-Saclay)
Lacroix, Thomas (Université Paris-Saclay)
Domínguez, María Ángeles (Instituto de Salud Carlos III)
Leblond-Bourget, Nathalie (Université de Lorraine)
Ardanuy, Carmen (Universitat de Barcelona)

Fecha:	2023
Resumen:	Tetracycline resistance in streptococci is mainly due to ribosomal protection mediated by the tet (M) gene that is usually located in the integrative and conjugative elements (ICEs) of the Tn 916 -family. In this study, we analyzed the genes involved in tetracycline resistance and the associated mobile genetic elements (MGEs) in Streptococcus dysgalactiae subsp. equisimilis (SDSE) causing invasive disease. SDSE resistant to tetracycline collected from 2012 to 2019 in a single hospital and from 2018 in three other hospitals were analyzed by whole genome sequencing. Out of a total of 84 SDSE isolates, 24 (28. 5%) were resistant to tetracycline due to the presence of tet (M) (n = 22), tet (W) (n = 1), or tet (L) plus tet (W) (n = 1). The tet (M) genes were found in the ICEs of the Tn 916 -family (n = 10) and in a new integrative and mobilizable element (IME; n = 12). Phylogenetic analysis showed a higher genetic diversity among the strains carrying Tn 916 than those having the new IME, which were closely related, and all belonged to CC15. In conclusion, tetracycline resistance in SDSE is mostly due to the tet (M) gene associated with ICEs belonging to the Tn 916 -family and a new IME. This new IME is a major cause of tetracycline resistance in invasive Streptococcus dysgalactiae subsp. equisimilis in our settings.
Ayudas:	Instituto de Salud Carlos III CB21/13/0009 Instituto de Salud Carlos III PI21/01000 Ministerio de Sanidad y Consumo CB06/06/0037
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Streptococcus dysgalactiae subsp. equisimilis ; Tet (M) ; IME ; ICE ; Mobile genetic elements
Publicado en:	Antibiotics, Vol. 12, Num. 3 (March 2023) , art. 579, ISSN 2079-6382

DOI: 10.3390/antibiotics12030579
PMID: 36978446

8 p, 971.4 KB

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