TEMPRANILLO homologs in apple regulate flowering time in the woodland strawberry Fragaria vesca
Dejahang, Ata (National Institute of Genetic Engineering and Biotechnology. Department of Plant Biotechnology (Iran))
Maghsoudi, Naeimeh (University of Tabriz. Department of Horticultural Sciences)
Mousavi, Amir (National Institute of Genetic Engineering and Biotechnology. Department of Plant Biotechnology (Iran))
Farsad-Akhtar, Nader (University of Tabriz. Department of Plant Biology)
Matias-Hernandez, Luis (Centre de Recerca en Agrigenòmica)
Pelaz, Soraya (Centre de Recerca en Agrigenòmica)
Folta, Kevin (University of Florida. Horticultural Sciences Department)
Mahna, Nasser (Centre de Recerca en Agrigenòmica)

Date:	2023
Abstract:	The long juvenile period of fruit trees makes their breeding costly and time-consuming. Therefore, flowering time engineering and shortening the juvenile phase have become a breeding priority for the genetic improvement of fruit tree crops. Many economically valuable fruit trees belong to the Rosaceae family including apples and strawberries. TEMPRANILLO (TEM) acts as a key player in flowering time control through inhibiting FT function. Two genes with high sequence similarity with the Arabidopsis TEM genes were isolated from apple (Malus domestica). Due to the complexity of carrying out functional studies in apple, we characterized their function in woodland strawberry as well as their expression in apple. The expression of MdTEM genes in apple tissues from juvenile plants was dramatically higher than that in the tissues from adult trees. In woodland strawberry, the overexpression of MdTEM genes down-regulated FvFT1, FvGA3OX1, and FvGA3OX2 genes in strawberry. The MdTEM -overexpressing lines exhibited delayed flowering, in terms of days to flowering and the number of leaves at flowering. While, RNAi-mediated silencing of TEM resulted in five days earlier flowering, with a lower number of leaves, a higher trichome density, and in some cases, caused in vitro flowering. According to these results and in silico analyses, it can be concluded that MdTEM1 and MdTEM2 can be considered as orthologs of FvTEM and probably AtTEM genes, which play an important role in regulating the juvenile phase and flowering time through regulating FT and GA biosynthetic pathway.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Biotechnology ; Genetics ; Molecular biology ; Plant sciences
Published in:	Scientific reports, Vol. 13 (February 2023) , art. 1968, ISSN 2045-2322

DOI: 10.1038/s41598-023-29059-0
PMID: 36737641

13 p, 3.4 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
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