Usefulness of Oral Fluid for Measurement of Methylone and Its Metabolites : Correlation with Plasma Drug Concentrations and the Effect of Oral Fluid pH
Sprega, Giorgia (Università Politecnica delle Marche)
Di Giorgi, Alessandro (Università Politecnica delle Marche)
Poyatos, Lourdes (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Papaseit, Esther (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Pérez-Mañá, Clara (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Tini, Anastasio (Università Politecnica delle Marche)
Pichini, Simona (Istituto Superiore di Sanità)
Busardo, Francesco Paolo (Università Politecnica delle Marche)
Lo Faro, Alfredo Fabrizio (Università Politecnica delle Marche)
Farre, Magi (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Universitat Autònoma de Barcelona

Date:	2023
Abstract:	The aim of this study was to investigate methylone and its metabolites concentration in oral fluid following controlled increasing doses, focusing on the effect of oral fluid pH. Samples were obtained from a clinical trial where twelve healthy volunteers participated after ingestion of 50, 100, 150 and 200 mg of methylone. Concentration of methylone and its metabolites 4-hydroxy-3-methoxy-N-methylcathinone (HMMC) and 3,4-methylenedioxycathinone in oral fluid were measured using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Pharmacokinetic parameters were estimated, and the oral fluid-to-plasma ratio (OF/P) at each time interval was calculated and correlated with the oral fluid pH using data from our previous study in plasma. Methylone was detected at all time intervals after each dose; MDC and HMMC were not detectable after the lowest dose. Oral fluid concentrations of methylone ranged between 88. 3-503. 8, 85. 5-5002. 3, 182. 8-13,201. 8 and 214. 6-22,684. 6 ng/mL following 50, 100, 150 and 200 mg doses, respectively, peaked between 1. 5 and 2. 0 h, and were followed by a progressive decrease. Oral fluid pH was demonstrated to be affected by methylone administration. Oral fluid is a valid alternative to plasma for methylone determination for clinical and toxicological studies, allowing for a simple, easy and non-invasive sample collection.
Grants:	Instituto de Salud Carlos III PI17/01962 Instituto de Salud Carlos III PI20/00879
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Humans ; LC-MS/MS ; Methylone ; Oral fluid ; Pharmacokinetics
Published in:	Metabolites, Vol. 13 (march 2023) , ISSN 2218-1989

DOI: 10.3390/metabo13040468
PMID: 37110127

12 p, 1.5 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Articles > Research articles
Articles > Published articles