Detection and genetic characterization of enteric viruses in diarrhoea outbreaks from swine farms in Spain
Puente, Héctor (Universidad de León. Departamento de Sanidad Animal)
Arguello, Héctor (Universidad de León. INDEGSAL)
Cortey, Martí (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Gómez-García, Manuel (Universidad de León. Departamento de Sanidad Animal)
Mencía-Ares, Oscar (Universidad de León. Departamento de Sanidad Animal)
Pérez-Perez, Lucía (Universidad de León. Departamento de Sanidad Animal)
Díaz, Ivan (Unitat mixta d'investigació IRTA-UAB en Sanitat Animal. Centre de Recerca en Sanitat Animal)
Carvajal, Ana (Universidad de León. INDEGSAL)

Data:	2023
Resum:	The aim of this work was to study the prevalence and distribution of Porcine astrovirus (PAstV), Porcine kobuvirus (PKoV), Porcine torovirus (PToV), Mammalian orthoreovirus (MRV) and Porcine mastadenovirus (PAdV) as well as their association with widely recognized virus that cause diarrhoea in swine such as coronavirus (CoVs) and rotavirus (RVs) in diarrhoea outbreaks from Spanish swine farms. Furthermore, a selection of the viral strains was genetically characterized. PAstV, PKoV, PToV, MRV and PAdV were frequently detected. Particularly, PAstV and PKoV were detected in almost 50% and 30% of the investigated farms, respectively, with an age-dependent distribution; PAstV was mainly detected in postweaning and fattening pigs, while PKoV was more frequent in sucking piglets. Viral co-infections were detected in almost half of the outbreaks, combining CoVs, RVs and the viruses studied, with a maximum of 5 different viral species reported in three investigated farms. Using a next generation sequencing approach, we obtained a total of 24 ARN viral genomes (> 90% genome sequence), characterizing for first time the full genome of circulating strains of PAstV2, PAstV4, PAstV5 and PToV on Spanish farms. Phylogenetic analyses showed that PAstV, PKoV and PToV from Spanish swine farms clustered together with isolates of the same viral species from neighboring pig producing countries. Although further studies to evaluate the role of these enteric viruses in diarrhoea outbreaks are required, their wide distribution and frequent association in co-infections cannot be disregard. Hence, their inclusion into routine diagnostic panels for diarrhoea in swine should be considered. The online version contains supplementary material available at 10. 1186/s40813-023-00326-w.
Ajuts:	Ministerio de Ciencia y Tecnología E-RTA2015-0003-C02-02 Ministerio de Ciencia y Tecnología E-RTA2015-0003-C02-01 Ministerio de Ciencia y Tecnología FPU17/00466 Ministerio de Ciencia y Tecnología FPU16/03485 Ministerio de Ciencia y Tecnología PRE2020-093762 Ministerio de Ciencia y Tecnología RYC-2015-1715-4 Ministerio de Ciencia y Tecnología BEAGAL- 18-106
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Swine ; Multiplex RT-PCR ; Phylogenetic analysis ; Porcine astrovirus ; Porcine kobuvirus ; Porcine torovirus ; Mammalian orthoreovirus ; Porcine mastadenovirus
Publicat a:	Porcine health management, Vol. 9 (june 2023) , ISSN 2055-5660

DOI: 10.1186/s40813-023-00326-w
PMID: 37349807

14 p, 2.1 MB

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