Hybrid Gold Nanorod-Based Nanoplatform with Chemo and Photothermal Activities for Bimodal Cancer Therapy
Arellano-Galindo, Lilia (Universidade de Santiago de Compostela. Departamento de Física de Partículas)
Villar-Alvarez, Eva (Institut Català de Nanociència i Nanotecnologia)
Varela, Alejandro (Universidade de Santiago de Compostela. Departamento de Física de Partículas)
Figueroa, Valeria (Universidad de Guadalajara. Departamento de Ingeniería Química)
Fernandez-Vega, Javier (Universidade de Santiago de Compostela. Departamento de Física de Partículas)
Cambón, Adriana (Universidade de Santiago de Compostela. Departamento de Física de Partículas)
Prieto, Gerardo (Universidade de Santiago de Compostela. Departamento de Física Aplicada)
Barbosa, Silvia (Universidade de Santiago de Compostela. Departamento de Física de Partículas)
Taboada, Pablo (Universidade de Santiago de Compostela. Departamento de Física de Partículas)

Data:	2022
Resum:	Metal nanoparticles (NPs), particularly gold nanorods (AuNRs), appear as excellent platforms not only to transport and deliver bioactive cargoes but also to provide additional therapeutic responses for diseased cells and tissues and/or to complement the action of the carried molecules. In this manner, here, we optimized a previous developed metal-based nanoplatform composed of an AuNR core surrounded by a polymeric shell constructed by means of the layer-by-layer approach, and in which very large amounts of the antineoplasic drug doxorubicin (DOXO) in a single loading step and targeting capability thanks to an outer hyaluronic acid layer were incorporated by means of an optimized fabrication process (PSS/DOXO/PLL/HA-coated AuNRs). The platform retained its nanometer size with a negative surface charge and was colloidally stable in a range of physiological conditions, in which only in some of them some particle clustering was noted with no precipitation. In addition, the dual stimuli-responsiveness of the designed nanoplatform to both endogenous proteases and external applied light stimuli allows to perfectly manipulate the chemodrug release rates and profiles to achieve suitable pharmacodynamics. It was observed that the inherent active targeting abilities of the nanoplatfom allow the achievement of specific cell toxicity in tumoral cervical HeLa cells, whilst healthy ones such as 3T3-Balb fibroblast remain safe and alive in agreement with the detected levels of internalization in each cell line. In addition, the bimodal action of simultaneous chemo- and photothermal bioactivity provided by the platform largely enhances the therapeutic outcomes. Finally, it was observed that our PSS/DOXO/PLL/HA-coated AuNRs induced cell mortality mainly through apoptosis in HeLa cells even in the presence of NIR light irradiation, which agrees with the idea of the chemo-activity of DOXO predominating over the photothermal effect to induce cell death, favoring an apoptotic pathway over necrosis for cell death.
Ajuts:	Agencia Estatal de Investigación PID2019-109517RB-I00
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Gold nanorods ; Layer assembly ; Photothermal therapy ; Multimodal therapy ; Cell death pathway
Publicat a:	International journal of molecular sciences, Vol. 23, Issue 21 (October 2022) , art. 13109, ISSN 1422-0067

DOI: 10.3390/ijms232113109
PMID: 36361892

25 p, 5.2 MB

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