Evaluation of Fourier Transform Infrared Spectroscopy as a First-Line Typing Tool for the Identification of Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae Outbreaks in the Hospital Setting
Wang-Wang, Jun Hao (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Bordoy, Antoni E. (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Martró, Elisa (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Quesada, María Dolores (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Pérez-Vázquez, María (Instituto de Salud Carlos III)
Guerrero Murillo, Mercedes (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Tiburcio, Andrea (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Navarro, Marina (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Castellà, Laia (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Sopena, Nieves (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Casas, Irma (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Saludes, Verónica (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Giménez, Montserrat (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Cardona, Pere-Joan (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Fecha:	2022
Resumen:	Early detection of pathogen cross-transmission events and environmental reservoirs is needed to control derived nosocomial outbreaks. Whole-genome sequencing (WGS) is considered the gold standard for outbreak confirmation, but, in most cases, it is time-consuming and has elevated costs. Consequently, the timely incorporation of WGS results to conventional epidemiology (CE) investigations for rapid outbreak detection is scarce. Fourier transform infrared spectroscopy (FTIR) is a rapid technique that establishes similarity among bacteria based on the comparison of infrared light absorption patterns of bacterial polysaccharides and has been used as a typing tool in recent studies. The aim of the present study was to evaluate the performance of the FTIR as a first-line typing tool for the identification of extended-spectrum β-lactamase-producing Klebsiella pneumoniae (ESBL-Kp) outbreaks in the hospital setting in comparison with CE investigations using WGS as the gold standard method. Sixty-three isolates of ESBL-Kp collected from 2018 to 2021 and classified according to CE were typed by both FTIR and WGS. Concordance was measured using the Adjusted Rand index (AR) and the Adjusted Wallace coefficient (AW) for both CE and FTIR clustering considering WGS as the reference method. Both AR and AW were significantly higher for FTIR clustering than CE clustering (0. 475 vs. 0. 134, p = 0. 01, and 0. 521 vs. 0. 134, p = 0. 009, respectively). Accordingly, FTIR inferred more true clustering relationships than CE (38/42 vs. 24/42, p = 0. 001). However, a similar proportion of genomic singletons was detected by both FTIR and CE (13/21 vs. 12/21, p = 1). This study demonstrates the utility of the FTIR method as a quick, low-cost, first-line tool for the detection of ESBL-Kp outbreaks, while WGS analyses are being performed for outbreak confirmation and isolate characterization. Thus, clinical microbiology laboratories would benefit from integrating the FTIR method into CE investigations for infection control measures in the hospital setting.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Cluster analysis ; Conventional epidemiology ; Fourier transform infrared spectroscopy ; FTIR ; Klebsiella pneumoniae ; Nosocomial infection ; Outbreak ; Whole-genome sequencing
Publicado en:	Frontiers in microbiology, Vol. 13 (June 2022) , art. 897161, ISSN 1664-302X

DOI: 10.3389/fmicb.2022.897161
PMID: 35756036

12 p, 2.4 MB

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