Modulation of the autophagic pathway inhibits HIV-1 infection in human lymphoid tissue cultured ex vivo
Pedreño López, Sònia (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa)
García Rodríguez, Elisabet (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa)
Guerrero, Dolores (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Gomez, Elisabet (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa)
Molina Mateu, Laura (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Orera Pérez, Fernando (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Senserrich, Jordi (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa)
Clotet Sala, Bonaventura (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa)
Cabrera Navarro, Cecilia (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa)
Universitat Autònoma de Barcelona. Departament de Cirurgia

Date:	2022
Abstract:	A complex link exists between HIV-1 and autophagy, and discordant results have been reported in different in vitro models regarding the way HIV and autophagy modulate each other. Despite this, there is very limited knowledge about the interplay between HIV and autophagy in vivo in lymphoid tissue, due in part by the lack of cell models that recapitulate the in vivo setting. Here, we evaluate the interrelationship between HIV and autophagy using human ex vivo lymphoid tissue cultures as an HIV infection model. Our results showed that human lymphoid aggregated cultures (HLACs) from tonsillar tissue displayed fully functional autophagic activity. In this system, HIV infection resulted in an increase in autophagy. Notably, we observed that both, autophagy-enhancing (rapamycin) or blocking drugs (3-methyladenine, chloroquine and bafilomycin), were able to decrease HIV-DNA levels and HIV replication. Therefore, efficient HIV-1 replication requires a fine-tuned level of autophagy, so modifications of this balance will have a negative impact on its replication. Therefore, targeting the autophagic pathway could be a new therapeutic approach to be explored to treat HIV-1 infection. Ex vivo cultures of human lymphoid tissue are a suitable model to obtain further insights into HIV and its intricate relationship with autophagy.
Grants:	Ministerio de Economía y Competitividad PI12/02408 Ministerio de Economía y Competitividad PI15/01053 Instituto de Salud Carlos III PI18/01226
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	HIV infections ; Macroautophagy
Published in:	Scientific reports, Vol. 12 (may 2022) , ISSN 2045-2322
Related work:	Pedreño‑López, Sònia; García Rodríguez, Elisabet; Guerrero, Dolores; [et al.]. «Author Correction : Modulation of the autophagic pathway inhibits HIV-1 infection in human lymphoid tissue cultured ex vivo». Scientific reports, Vol. 13 (february 2023) https://doi.org/10.1038/s41598-023-30114-z

Correcció de l'article: https://ddd.uab.cat/record/272467
DOI: 10.1038/s41598-022-11181-0
PMID: 35523829

11 p, 2.5 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Articles > Research articles
Articles > Published articles