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| Pàgina inicial > Articles > Articles publicats > Lung Extracellular Matrix Hydrogels Enhance Preservation of Type II Phenotype in Primary Alveolar Epithelial Cells |
(Centro de Investigación Biomédica en Red de Enfermedades Raras) (Universitat de Barcelona) (Parc Taulí Hospital Universitari. Institut d'Investigació i Innovació Parc Taulí (I3PT)) (Institut d'Investigacions Biomèdiques August Pi i Sunyer) (Universitat de Barcelona) (Parc Taulí Hospital Universitari. Institut d'Investigació i Innovació Parc Taulí (I3PT)) (Institut d'Investigacions Biomèdiques August Pi i Sunyer)| Data: | 2022 |
| Resum: | One of the main limitations of in vitro studies on lung diseases is the difficulty of maintaining the type II phenotype of alveolar epithelial cells in culture. This fact has previously been related to the translocation of the mechanosensing Yes-associated protein (YAP) to the nuclei and Rho signaling pathway. In this work, we aimed to culture and subculture primary alveolar type II cells on extracellular matrix lung-derived hydrogels to assess their suitability for phenotype maintenance. Cells cultured on lung hydrogels formed monolayers and maintained type II phenotype for a longer time as compared with those conventionally cultured. Interestingly, cells successfully grew when they were subsequently cultured on a dish. Moreover, cells cultured on a plate showed the active form of the YAP protein and the formation of stress fibers and focal adhesions. The results of chemically inhibiting the Rho pathway strongly suggest that this is one of the mechanisms by which the hydrogel promotes type II phenotype maintenance. These results regarding protein expression strongly suggest that the chemical and biophysical properties of the hydrogel have a considerable impact on the transition from ATII to ATI phenotypes. In conclusion, culturing primary alveolar epithelial cells on lung ECM-derived hydrogels may facilitate the prolonged culturing of these cells, and thus help in the research on lung diseases. |
| Ajuts: | Agencia Estatal de Investigación SAF2017-85574-R Agencia Estatal de Investigación DPI2017-83721-P Agencia Estatal de Investigación PID2019-108958RB-I00 Agencia Estatal de Investigación PGC2018-097323-A-I00 |
| Drets: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.  |
| Llengua: | Anglès |
| Document: | Article ; recerca ; Versió publicada |
| Matèria: | Alveolar cells ; Extracellular matrix ; Hydrogels ; Type II phenotype ; YAP |
| Publicat a: | International journal of molecular sciences, Vol. 23 (april 2022) , ISSN 1422-0067 |
