Cytotoxic NK cells phenotype and activated lymphocytes are the main characteristics of patients with alcohol-associated liver disease
Zurera Egea, Coral (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Teniente Serra, Aina (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Fuster, Daniel (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Martínez Cáceres, Eva María (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Muga, Roberto (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Zuluaga Blanco, Yenny Paola (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Universitat Autònoma de Barcelona

Data:	2023
Resum:	T cells, natural killer (NK) and NKT cells have opposing actions in the development of alcohol-associated liver fibrosis. We aimed to evaluate the phenotype of NK cells, NKT cells and activated T cells in patients with alcohol use disorder (AUD) according to the presence of advanced liver fibrosis (ALF). Totally, 79 patients (51-years, 71% males) were admitted to treatment of AUD. ALF was defined as FIB4-score > 2. 67. Immunophenotyping of NK cells (CD3CD56CD16, CD3CD56CD16, CD3CD56CD16), NKT-like (CD3CD56), and the activation status of CD4, CD8 and regulatory T cells (Tregs) were evaluated according to the HLA-DR expression. Patients had an AUD duration of 18 ± 11 years with a daily alcohol consumption of 155 ± 77 gr/day prior to hospital admission. The values of absolute cells were 2 ± 0. 9 cells/L for total lymphocytes, 1054 ± 501 cells/µL for CD4, 540 ± 335 cells/µL for CD8, 49. 3 ± 24. 8 cells/µL for Tregs, 150. 3 ± 97. 5 cells/µL for NK cells and 69. 8 ± 78. 3 cells/µL for NKT-like. The percentage of total NK cells (11. 3 ± 5. 5% vs. 7 ± 4. 3%, p < 0. 01), CD3CD56CD16 regarding total lymphocytes (9. 7 ± 5. 1% vs. 5. 8 ± 3. 9%, p < 0. 01), activated CD4 cells (5. 2 ± 3. 2% vs. 3. 9 ± 3%, p = 0. 04) and activated CD8 cells (15. 7 ± 9. 1% vs. 12. 2 ± 9%, p = 0. 05) were significantly higher in patients with ALF. The percentage of CD3CD56CD16 regarding NK cells (5. 1 ± 3. 4% vs. 7. 6 ± 6. 2%, p = 0. 03) was significantly lower in patients with ALF. Activated Tregs (39. 9 ± 11. 5 vs. 32. 4 ± 9. 2, p = 0. 06) showed a tendency to be higher in patients with ALF. The proportion of activated CD4 cells (r = 0. 40, p < 0. 01) and activated CD8 cells (r = 0. 51, p < 0. 01) was correlated with the proportion of NKT-like in patients without ALF. Patients with ALF presented an increased NK cytotoxic phenotype and activated T cells concomitant with a decreased NK cytokine-secreting phenotype.
Ajuts:	Instituto de Salud Carlos III RD21/0009/0004 Instituto de Salud Carlos III PI20/00883
Nota:	Altres ajuts: acords transformatius de la UAB
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Cytotoxic NK cells ; Activated T cells ; NKT-like ; Alcohol-associated liver fbrosis
Publicat a:	Clinical and Experimental Medicine, Vol. 23, Num. 7 (november 2023) , p. 3539-3547, ISSN 1591-9528

DOI: 10.1007/s10238-023-01121-1
PMID: 37392250

9 p, 1.0 MB

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Documents de recerca > Documents dels grups de recerca de la UAB > Centres i grups de recerca (producció científica) > Ciències de la salut i biociències > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
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