Polyhydroxyalkanoates production by an advanced food-on-demand strategy : The effect of operational conditions
Mineo, Antonio (Palermo University. Engineering Department)
Isern-Cazorla, Laura (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Rizzo, Carla (Palermo University. Department of Biological)
Piccionello, Antonio Palumbo (Palermo University. Department of Biological)
Suárez Ojeda, María Eugenia (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Mannina, Giorgio (Palermo University. Engineering Department)

Fecha:	2023
Resumen:	Despite the increasing number of studies related to the polyhydroxyalkanoate (PHA) production from sewage sludge of wastewater treatment plants, there is still a gap in the correlation between the operational conditions, such as the organic loading rate (OLR), and the intracellular polyhydroxyalkanoate (PHA) content, productivity and final recovery of the polymer. Therefore, this work aims to provide experimental data on PHA productivity and purity in view of scaling up the process to an industrial level taking into account process parameters. In view of that, three OLR were applied during the selection of PHA-accumulating bacteria in sewage sludge. Then, the biomass was harvested and subjected to batch accumulation experiments at two organic loads per dosage by employing a tailor-made software to adopt an automated feed-on-demand strategy, which allowed for 30-56 h of accumulation tests in stand-alone mode. Finally, an improved protocol for PHA extraction has been applied. Experimental results show that the maximum PHA content (60% w/w) was achieved using the highest organic load per dosage during the accumulation test with the biomass selected at the highest OLR (1. 8 g COD L d). Also, the extraction protocol efficiency was proven with four samples with different PHA content, achieving recovery yield as high as 78 ± 3 % with a purity of 89 ± 2 %, thus demonstrating that the adopted strategy might be beneficial for industrial use.
Ayudas:	European Commission 869283 Agencia Estatal de Investigación PID2021-126102OB-I00
Nota:	Altres ajuts: acords transformatius de la UAB
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Automated accumulation software ; Bioplastic production ; Biopolymers extraction ; Water resource recovery
Publicado en:	Chemical engineering journal, Vol. 472 (September 2023) , art. 145007, ISSN 1873-3212

DOI: 10.1016/j.cej.2023.145007

9 p, 1.1 MB

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