Tetracycline resistance transmission in Campylobacter is promoted at temperatures resembling the avian reservoir
Cuevas-Ferrando, E. (Universitat de Barcelona)
Guirado, Pedro (Universitat de Barcelona)
Miró, Elisenda (Institut d'Investigació Biomèdica Sant Pau)
Iglesias Torrens, Yaidelys (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Navarro Risueño, Ferran (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Alioto, Tyler Scott (Universitat Pompeu Fabra)
Gómez-Garrido, Jessica (Centre de Regulació Genòmica)
Madrid Xufré, Cristina (Universitat de Barcelona)
Balsalobre Parra, Carlos (Universitat de Barcelona)

Data:	2020
Resum:	Campylobacter is the causal agent of campylobacteriosis in humans, a self-limiting gastroenteritis. Campylobacteriosis is a zoonosis, commonly transmitted from contaminated chicken meat by either direct consumption or cross contamination during food manipulation. Presence of plasmids encoding for resistance to antibiotics such as tetracycline is common among Campylobacter isolates. In this report, we studied the effect of the temperature in the conjugation frequency of several tet(O) carrying plasmids, providing tetracycline resistance to the recipient cells. The conjugation frequency from donor cells carrying three previously characterized plasmids (pCjA13, pCjA9 and pTet) and from two clinical isolates was determined. Two temperatures, 37 and 42 °C, mimicking the conditions encountered by C. jejuni in the human and broiler chicken gastrointestinal tracts, respectively, were assessed. Our results clearly indicate that the conjugation process is promoted at high temperature. Accordingly, the transcriptional expression of some putative conjugative apparatus genes is thermoregulated, being induced at 42 °C. The two plasmids present in the clinical isolates were sequenced and assembled. Both plasmids are highly related among them and to the pTet plasmid. The high identity of the genes putatively involved in the conjugation process among the plasmids is in agreement with the similar behavior regarding the temperature dependency of the conjugative process. This report suggest that conjugation of plasmids carrying antibiotic resistance genes occurs preferentially at temperatures that resemble the gastrointestinal tract of birds, the main reservoir of C. jejuni.
Ajuts:	Ministerio de Economía y Competitividad AGL2103-45339R Agencia Estatal de Investigación PGC2018-096958-B-I00 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-499
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Llengua:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Matèria:	Campylobacter ; Plasmid conjugation ; Temperature regulation ; Tetracycline resistance
Publicat a:	Veterinary Microbiology, Vol. 244 (May 2020) , art. 108652, ISSN 1873-2542

DOI: 10.1016/j.vetmic.2020.108652
PMID: 32402330

Postprint 39 p, 2.7 MB

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