CkP1 bacteriophage, a S16-like myovirus that recognizes Citrobacter koseri lipopolysaccharide through its long tail fibers
Oliveira, Hugo (LABBELS -Associate Laboratory)
Santos, Sílvio (LABBELS -Associate Laboratory)
Pires, Diana P. (LABBELS -Associate Laboratory)
Boeckaerts, Dimitri (Ghent University)
Pinto, Graça (LABBELS -Associate Laboratory)
Domingues, Rita (LABBELS -Associate Laboratory)
Otero Carrera, Jennifer (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Briers, Yves (Ghent University)
Lavigne, Rob (KU Leuven)
Schmelcher, Mathias (ETH Zurich)
Dötsch, Andreas (Max Rubner-Institute)
Azeredo, Joana (LABBELS -Associate Laboratory)

Data:	2023
Resum:	Citrobacter koseri is an emerging Gram-negative bacterial pathogen, which causes urinary tract infections. We isolated and characterized a novel S16-like myovirus CKP1 (vB_CkoM_CkP1), infecting C. koseri. CkP1 has a host range covering the whole C. koseri species, i. e. , all strains that were tested, but does not infect other species. Its linear 168,463-bp genome contains 291 coding sequences, sharing sequence similarity with the Salmonella phage S16. Based on surface plasmon resonance and recombinant green florescence protein fusions, the tail fiber (gp267) was shown to decorate C. koseri cells, binding with a nanomolar affinity, without the need of accessory proteins. Both phage and the tail fiber specifically bind to bacterial cells by the lipopolysaccharide polymer. We further demonstrate that CkP1 is highly stable towards different environmental conditions of pH and temperatures and is able to control C. koseri cells in urine samples. Altogether, CkP1 features optimal in vitro characteristics to be used both as a control and detection agent towards drug-resistant C. koseri infections. • CkP1 infects all C. koseri strains tested • CkP1 recognizes C. koseri lipopolysaccharide through its long tail fiber • Both phage CkP1 and its tail fiber can be used to treat or detect C. koseri pathogens The online version contains supplementary material available at 10. 1007/s00253-023-12547-8.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Citrobacter ; Bacterial infection ; Bacteriophage ; Long tail fiber ; Control ; Diagnostics
Publicat a:	Applied Microbiology and Biotechnology, Vol. 107 (May 2023) , p. 3621-3636, ISSN 1432-0614

DOI: 10.1007/s00253-023-12547-8
PMID: 37133800

16 p, 1.4 MB

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