Evaluation of in vitro intrinsic radiosensitivity and characterization of five canine high-grade glioma cell lines
Cartiaux, Benjamin (Université de Toulouse. Toulouse NeuroImaging Center)
Deviers, Alexandra (Université de Toulouse. Toulouse NeuroImaging Center)
Delmas, Caroline (IUCT-oncopole)
Abadie, Jérôme (Laboniris. Department of Biology)
Pumarola i Batlle, Martí (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Cohen-Jonathan Moyal, Elizabeth (IUCT-oncopole)
Mogicato, Giovanni (Université de Toulouse. Toulouse NeuroImaging Center)

Date:	2023
Abstract:	Glioma is the most common primary brain tumor in dogs and predominantly affects brachycephalic breeds. Diagnosis relies on CT or MRI imaging, and the proposed treatments include surgical resection, chemotherapy, and radiotherapy depending on the tumor's location. Canine glioma from domestic dogs could be used as a more powerful model to study radiotherapy for human glioma than the murine model. Indeed, (i) contrary to mice, immunocompetent dogs develop spontaneous glioma, (ii) the canine brain structure is closer to human than mice, and (iii) domestic dogs are exposed to the same environmental factors than humans. Moreover, imaging techniques and radiation therapy used in human medicine can be applied to dogs, facilitating the direct transposition of results. The objective of this study is to fully characterize 5 canine glioma cell lines and to evaluate their intrinsic radiosensitivity. Canine cell lines present numerous analogies between the data obtained during this study on different glioma cell lines in dogs. Cell morphology is identical, such as doubling time, clonality test and karyotype. Immunohistochemical study of surface proteins, directly on cell lines and after stereotaxic injection in mice also reveals close similarity. Radiosensitivity profile of canine glial cells present high profile of radioresistance.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Glioma ; Canine ; Characterization ; Translational ; Radiation
Published in:	Frontiers in Veterinary Science, Vol. 10 (november 2023) , ISSN 2297-1769

DOI: 10.3389/fvets.2023.1253074
PMID: 38098992

10 p, 1.1 MB

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