Programmed death-ligand (PD-L1), epidermal growth factor (EGF), relaxin and metalloproteinase-3 (MMP3), potential biomarkers of malignancy in canine mammary neoplasia
Galadima, Makchit
Teles, Mariana (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Pastor Milán, Josep (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Hernandez-Losa, Javier (Hospital Universitari Vall d'Hebron)
Rodríguez Gil, Joan Enric (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Rivera del Álamo, Maria Montserrat (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)

Date:	2024
Description:	15 pàg.
Abstract:	Gene expression has been suggested as a putative tool for prognosis and diagnosis in canine mammary neoplasia (CMNs). In the present study, 58 formalin-fixed paraffin-embedded (FFPE) paraffined canine mammary neoplasias from 27 different bitches were included. Thirty-seven tumours were classified as benign, whereas thirty-one were classified as different types of canine carcinoma. In addition, mammary samples from three healthy bitches were also included. The gene expression for vascular endothelial growth factor-α (VEGFα), CD20, progesterone receptor (PGR), hyaluronidase-1 (HYAL-1), programmed death-ligand 1 (PD-L1), epidermal growth factor (EGF), relaxin (RLN2), and matrix metalloproteinase-3 (MMP3) was assessed through RT-qPCR. All the assessed genes yielded a higher expression in neoplastic mammary tissue than in healthy tissue. All the evaluated genes were overexpressed in neoplastic mammary tissue, suggesting a role in the process of tumorigenesis. Moreover, PD-L1, EGF, relaxin, and MMP3 were significantly overexpressed in malignant CMNs compared to benign CMNs, suggesting they may be useful as malignancy biomarkers.
Grants:	Agencia Estatal de Investigación PID 2020-113221RB-I00 Ministerio de Ciencia e Innovación RYC 2019-026841-I
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Canine mammary neoplasia ; Gene expression ; Malignancy biomarkers ; Programmed death-ligand 1 ; Epidermal growth factor ; Relaxin ; Matrix metalloprotease-3
Published in:	International journal of molecular sciences, Vol. 25 (2024) , p. 1170, ISSN 1422-0067

DOI: 10.3390/ijms25021170
PMID: 38256245

15 p, 1.2 MB

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