Cross-contamination of mature Listeria monocytogenes biofilms from stainless steel surfaces to chicken broth before and after the application of chlorinated alkaline and enzymatic detergents
Mazaheri, Tina (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Ripolles-Avila, Carolina (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Rodríguez-Jerez, José Juan (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)

Date:	2023
Description:	8 pàg.
Abstract:	The objectives of this study were, firstly, to compare a conventional (i. e. , chlorinated alkaline) versus an alternative (chlorinated alkaline plus enzymatic) treatment effectivity for the elimination of biofilms from different L. monocytogenes strains (CECT 5672, CECT 935, S2-bac and EDG-e). Secondly, to evaluate the cross-contamination to chicken broth from non-treated and treated biofilms formed on stainless steel surfaces. Results showed that all L. monocytogenes strains were able to adhere and develop biofilms at approximately the same growth levels (≈5. 82 log CFU/cm2). When non-treated biofilms were put into contact with the model food, obtained an average transference rate of potential global cross-contamination of 20. 4%. Biofilms treated with the chlorinated alkaline detergent obtained transference rates similar to non-treated biofilms as a high number of residual cells (i. e. , around 4 to 5 Log CFU/cm2) were present on the surface, except for EDG-e strain on which transference rate diminished to 0. 45%, which was related to the protective matrix. Contrarily, the alternative treatment was shown to not produce cross-contamination to the chicken broth due to its high effectivity for biofilm control (<0. 50% of transference) except for CECT 935 strain that had a different behavior. Therefore, changing to more intense cleaning treatments in the processing environments can reduce risk of cross-contamination.
Grants:	Ministerio de Ciencia, Innovación y Universidades RTI 2018-098267-R-C32
Note:	Altres ajuts: acords transformatius de la UAB
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Biofilms ; Cross-contamination ; Decontamination agents ; Food safety ; L. monocytogenes ; Stainless Steel/analysis ; Animals ; Chickens ; Listeria monocytogenes ; Colony Count, Microbial ; Detergents ; Food Microbiology
Published in:	FOOD MICROBIOLOGY, Vol. 112 (2023) , p. 104236, ISSN 1095-9998

DOI: 10.1016/j.fm.2023.104236
PMID: 36906320

8 p, 580.9 KB

The record appears in these collections:
Articles > Research articles
Articles > Published articles