Indirect competitive immunoassay for the detection of fungicide Thiabendazole in whole orange samples by Surface Plasmon Resonance
Estévez, M. Carmen (Institut Català de Nanociència i Nanotecnologia)
Belenguer, José (Ainia Centro Tecnológico (Valencia))
Gomez-Montes, Silvia (Institut Català de Nanociència i Nanotecnologia)
Miralles, Javier (Ainia Centro Tecnológico (Valencia))
Escuela, Alfonso M. (Universidad de Las Palmas de Gran Canaria)
Montoya, Angel (Universitat Politècnica de València)
Lechuga, Laura M. (Institut Català de Nanociència i Nanotecnologia)

Data:	2012
Resum:	A highly sensitive and specific SPR-based competitive immunoassay for the detection of Thiabendazole (TBZ) has been developed. An indirect format where a TBZ-protein conjugate is immobilized onto gold surfaces has been selected. Under the optimal conditions, a LOD of 0. 67 nM (0. 13 μg L) and an IC of 3. 2 nM (0. 64 μg L) have been achieved which are comparable to the values obtained by conventional ELISA. Analysis of real samples has been attempted by first evaluating the influence of complex matrix samples coming from whole oranges and secondly measuring samples containing TBZ previously evaluated by chromatographic methods. A methanolic extraction procedure followed by a simple dilution in assay buffer has proven to be sufficient to measure orange samples using the developed immunoassay with an excellent recovery percentage. The sensitivity and the feasibility of measuring whole orange samples demonstrate the effectiveness and robustness of the SPR biosensor, which can be useful for the determination of TBZ in food at concentrations below the Maximum Residue Levels (MRLs) established by the European legislation.
Nota:	Altres ajuts: Fundación M. Botín ; Instituto de la Mediana y Pequeña Industria Valenciana (IMPIVA) under the activity "Proyectos de alta especialización del personal investigador"
Drets:	Tots els drets reservats.
Llengua:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Publicat a:	Analyst, Vol. 137, Issue 23 (October 2012) , p. 5659-5665, ISSN 1364-5528

DOI: 10.1039/c2an36094b

Postprint 9 p, 894.5 KB

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