Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification
Rodriguez-Temporal, D. (Hospital General Universitario Gregorio Marañón)
Alcaide, Fernando (Hospital Universitari de Bellvitge)
Mareković, I. (University Hospital Centre Zagreb)
O'Connor, J.A. (Munster Technological University)
Gorton, R. (Health Services Laboratories)
van Ingen, J. (Radboud University Medical Centre)
Van den Bossche, A. (Sciensano)
Héry-Arnaud, G. (Hôpital Cavale Blanche)
Beauruelle, C. (Hôpital Cavale Blanche)
Orth-Höller, D. (Medical University of Innsbruck)
Palacios-Gutiérrez, J.J. (Hospital Universitario Central de Asturias)
Tudó, Griselda (Hospital Clínic i Provincial de Barcelona)
Bou, German (Hospital Universitario A Coruña)
Ceyssens, P.J. (Sciensano)
Garrigó, Montserrat (Institut d'Investigació Biomèdica Sant Pau)
Gonzalez-Martin, Julian (Hospital Clínic i Provincial de Barcelona)
Greub, G. (Université de Lausanne)
Hrabak, J. (Biomedical Center. Faculty of Medicine in Plzen)
Ingebretsen, A. (Oslo University Hospital (Oslo, Noruega))
Mediavilla-Gradolph, M.C. (Hospital Regional de Málaga)
Oviaño, M. (Hospital Universitario A Coruña)
Palop, B. (Hospital Regional de Málaga)
Pranada, A.B. (MVZ Dr. Eberhard & Partner Dortmund)
Quiroga, L. (Hospital General Universitario Gregorio Marañón)
Ruiz-Serrano, M.J. (Hospital General Universitario Gregorio Marañón)
Rodríguez-Sánchez, B. (Hospital General Universitario Gregorio Marañón)
Universitat Autònoma de Barcelona

Data:	2022
Resum:	The ability of MALDI-TOF for the identification of nontuberculous mycobacteria (NTM) has improved recently thanks to updated databases and optimized protein extraction procedures. Few multicentre studies on the reproducibility of MALDI-TOF have been performed so far, none on mycobacteria. The aim of this study was to evaluate the reproducibility of MALDI-TOF for the identification of NTM in 15 laboratories in 9 European countries. A total of 98 NTM clinical isolates were grown on Löwenstein-Jensen. Biomass was collected in tubes with water and ethanol, anonymized and sent out to the 15 participating laboratories. Isolates were identified using MALDI Biotyper (Bruker Daltonics). Up to 1330 MALDI-TOF identifications were collected in the study. A score ≥ 1. 6 was obtained for 100% of isolates in 5 laboratories (68. 2-98. 6% in the other). Species-level identification provided by MALDI-TOF was 100% correct in 8 centres and 100% correct to complex-level in 12 laboratories. In most cases, the misidentifications obtained were associated with closely related species. The variability observed for a few isolates could be due to variations in the protein extraction procedure or to MALDI-TOF system status in each centre. In conclusion, MALDI-TOF showed to be a highly reproducible method and suitable for its implementation for NTM identification.
Ajuts:	Ministerio de Economía y Competitividad PI15/01073 Instituto de Salud Carlos III PI18/00997 Instituto de Salud Carlos III PI18/01068
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Clinical microbiology ; Infectious-disease diagnostics
Publicat a:	Scientific reports, Vol. 12 Núm. 1 (december 2022) , p. 1237, ISSN 2045-2322

DOI: 10.1038/s41598-022-05315-7
PMID: 35075208

7 p, 1.0 MB

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