Gold Nanoparticles Modulate BCG-Induced Innate Immune Memory in Human Monocytes by Shifting the Memory Response towards Tolerance
Swartzwelter, Benjamin J. (Institute of Biochemistry and Cell Biology)
Barbero, Francesco (Institut Català de Nanociència i Nanotecnologia)
Verde, Alessandro (Institute of Biochemistry and Cell Biology)
Mangini, Maria (Institute of Biochemistry and Cell Biology)
Pirozzi, Marinella (Institute of Biochemistry and Cell Biology)
De Luca, Anna Chiara (Institute of Biochemistry and Cell Biology)
Puntes, Víctor (Institut Català de Nanociència i Nanotecnologia)
Leite, Luciana C. C. (Instituto Butantan. Laboratório de Desenvolvimento de Vacinas)
Italiani, Paola (Institute of Biochemistry and Cell Biology)
Boraschi, Diana (Institute of Biochemistry and Cell Biology)

Fecha:	2020
Resumen:	Innate immune memory is characterized by a modulation in the magnitude with which innate immune cells such as monocytes and macrophages respond to potential dangers, subsequent to previous exposure to the same or unrelated agents. In this study, we have examined the capacity of gold nanoparticles (AuNP), which are already in use for therapeutic and diagnostic purposes, to modulate the innate memory induced by bacterial agents. The induction of innate memory was achieved in vitro by exposing human primary monocytes to bacterial agents (lipopolysaccharide -LPS-, or live Bacille Calmette-Guérin -BCG) in the absence or presence of AuNP. After the primary activation, cells were allowed to return to a resting condition, and eventually re-challenged with LPS. The induction of memory was assessed by comparing the response to the LPS challenge of unprimed cells with that of cells primed with bacterial agents and AuNP. The response to LPS was measured as the production of inflammatory (TNFα, IL-6) and anti-inflammatory cytokines (IL-10, IL-1Ra). While ineffective in directly inducing innate memory per se, and unable to influence LPS-induced tolerance memory, AuNP significantly affected the memory response of BCG-primed cells, by inhibiting the secondary response in terms of both inflammatory and anti-inflammatory factor production. The reprogramming of BCG-induced memory towards a tolerance type of reactivity may open promising perspectives for the use of AuNP in immunomodulatory approaches to autoimmune and chronic inflammatory diseases.
Ayudas:	European Commission 671881 European Commission 812661
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Innate immune memory ; Gold nanoparticles ; Monocytes ; Potentiation ; BCG ; Inflammatory cytokines ; Anti-inflammatory cytokines
Publicado en:	Cells, Vol. 9, Issue 2 (February 2020) , art. 284, ISSN 2073-4409

DOI: 10.3390/cells9020284
PMID: 31979412

17 p, 1.5 MB

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