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1.
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26 p, 1.5 MB |
The Role of common cell culture media supplements on citrate-stabilized gold nanoparticle protein corona formation, aggregation state, and the consequent impact on cellular uptake
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Barbero, Francesco (Institut Català de Nanociència i Nanotecnologia) ;
Michelini, Sara (University of Salzburg. Department of Biosciences) ;
Moriones, Oscar Hernando (Universitat Autònoma de Barcelona) ;
Patarroyo, Javier (Institut Català de Nanociència i Nanotecnologia) ;
Rosell Aluja, Jordi 1955- (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Gustà, Muriel F (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Vitali, Michele (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Martín, Luna (Vall d'Hebron Institut d'Oncologia) ;
Canals, Francesc (Vall d'Hebron Institut d'Oncologia) ;
Duschl, Albert (University of Salzburg. Department of Biosciences) ;
Horejs-Hoeck, Jutta (University of Salzburg. Department of Biosciences) ;
Mondragón, Laura (Institut Català de Nanociència i Nanotecnologia) ;
Bastús, Neus G. (Institut Català de Nanociència i Nanotecnologia) ;
Puntes, Víctor (Institut Català de Nanociència i Nanotecnologia)
Sodium citrate-stabilized gold nanoparticles (AuNPs) are destabilized when dispersed in cell culture media (CCMs). This may promote their aggregation and subsequent sedimentation, or under the proper conditions, their interaction with dispersed proteins can lead to the formation of a NP-stabilizing protein corona. [...]
2022 -  10.1021/acs.bioconjchem.2c00232
Bioconjugate chemistry, Vol. 33, issue 8 (Aug. 2022) , p. 1505-1514
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2.
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13 p, 7.0 MB |
Innate memory reprogramming by gold nanoparticles depends on the microbial agents that induce memory
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Swartzwelter, Benjamin J. (Paris Lodron University of Salzburg. Department Biosciences) ;
Michelini, Sara (Paris Lodron University of Salzburg. Department Biosciences) ;
Frauenlob, Tobias (Paris Lodron University of Salzburg. Department Biosciences) ;
Barbero, Francesco (Institut Català de Nanociència i Nanotecnologia) ;
Verde, Alessandro (Consiglio Nazionale delle Ricerche (Nàpols, Itàlia). Istituto di Biochimica e Biologia Cellulare) ;
De Luca, Anna Chiara (Consiglio Nazionale delle Ricerche (Nàpols, Itàlia). Istituto di Biochimica e Biologia Cellulare) ;
Puntes, Víctor (Institut Català de Nanociència i Nanotecnologia) ;
Duschl, Albert (Paris Lodron University of Salzburg. Department Biosciences) ;
Horejs-Hoeck, Jutta (Paris Lodron University of Salzburg. Department Biosciences) ;
Italiani, Paola (Consiglio Nazionale delle Ricerche (Nàpols, Itàlia). Istituto di Biochimica e Biologia Cellulare) ;
Boraschi, Diana (Stazione Zoologica Anton Dohrn)
Innate immune memory, the ability of innate cells to react in a more protective way to secondary challenges, is induced by exposure to infectious and other exogeous and endogenous agents. Engineered nanoparticles are particulate exogenous agents that, as such, could trigger an inflammatory reaction in monocytes and macrophages and could therefore be also able to induce innate memory. [...]
2021 - 10.3389/fimmu.2021.751683
Frontiers in immunology, Vol. 12 (Nov. 2021) , art. 751683
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3.
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24 p, 2.6 MB |
Growth-promoting gold nanoparticles decrease stress responses in arabidopsis seedlings
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Ferrari, Eleonora (Universität Tübingen. Zentrum für Molekularbiologie der Pflanzen) ;
Barbero, Francesco (Institut Català de Nanociència i Nanotecnologia) ;
Busquets-Fité, Martí (Applied Nanoparticles. S.L.) ;
Franz-Wachtel, Mirita (c Proteom Centrum) ;
Köhler, Heinz-R. (Institut für Evolution und Ökologie. Institut für Evolution und Ökologie) ;
Puntes, Víctor (Institut Català de Nanociència i Nanotecnologia) ;
Kemmerling, Birgit (Universität Tübingen. Zentrum für Molekularbiologie der Pflanzen)
The global economic success of man-made nanoscale materials has led to a higher production rate and diversification of emission sources in the environment. For these reasons, novel nanosafety approaches to assess the environmental impact of engineered nanomaterials are required. [...]
2021 - 10.3390/nano11123161
Nanomaterials, Vol. 11, issue 12 (Dec. 2021) , art. 3161
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4.
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26 p, 2.5 MB |
Addressing nanomaterial immunosafety by evaluating innate immunity across living species
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Boraschi, Diana (Istituto di Biochimica e Biologia Cellulare (Nàpols)) ;
Alijagic, Andi (Istituto per la Ricerca e l'Innovazione Biomedica (Palermo)) ;
Auguste, Manon (Università di Genova. Dipartimento di scienze della terra, dell'ambiente e della vita) ;
Barbero, Francesco (Institut Català de Nanociència i Nanotecnologia) ;
Ferrari, Eleonora (ZMBP Eberhard-Karls University Tübingen. Center for Plant Molecular Biology) ;
Hernadi, Szabolcs (Cardiff University. School of Biosciences) ;
Mayall, Craig (University of Liubljana. Department of Biology) ;
Michelini, Sara (Paris-Lodron-University Salzburg. Department of Biosciences) ;
Navarro Pacheco, Natividad I. (Czech Academy of Sciences. Institute of Microbiology) ;
Prinelli, Alessandra (AvantiCell Science Ltd) ;
Swart, Elmer (UK Centre for Ecology and Hydrology) ;
Swartzwelter, Benjamin J. (Istituto di Biochimica e Biologia Cellulare (Nàpols)) ;
Bastús, Neus G. (Institut Català de Nanociència i Nanotecnologia) ;
Canesi, Laura (Università di Genova. Dipartimento di scienze della terra, dell'ambiente e della vita) ;
Drobne, Damjana (University of Liubljana. Department of Biology) ;
Duschl, Albert (Paris-Lodron-University Salzburg. Department of Biosciences) ;
Ewart, Marie-Ann (AvantiCell Science Ltd) ;
Horejs-Hoeck, Jutta (Paris-Lodron-University Salzburg. Department of Biosciences) ;
Italiani, Paola (Istituto di Biochimica e Biologia Cellulare (Nàpols)) ;
Kemmerling, Birgit (Center for Plant Molecular Biology - ZMBP. Eberhard-Karls University Tübingen) ;
Kille, Peter (Cardiff University. School of Biosciences) ;
Prochazkova, Petra (Czech Academy of Sciences. Institute of Microbiology) ;
Puntes, Víctor (Institut Català de Nanociència i Nanotecnologia) ;
Spurgeon, David J. (UK Centre for Ecology and Hydrology) ;
Svendsen, Claus (UK Centre for Ecology and Hydrology) ;
Wilde, Colin J. (AvantiCell Science Ltd) ;
Pinsino, Annalisa (Istituto per la Ricerca e l'Innovazione Biomedica (Palermo))
The interaction of a living organism with external foreign agents is a central issue for its survival and adaptation to the environment. Nanosafety should be considered within this perspective, and it should be examined that how different organisms interact with engineered nanomaterials (NM) by either mounting a defensive response or by physiologically adapting to them. [...]
2020 - 10.1002/smll.202000598
Small, Vol. 16, issue 21 (May 2020) , art. 2000598
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5.
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17 p, 10.0 MB |
Immunomodulatory Function of Polyvinylpyrrolidone (PVP)-Functionalized Gold Nanoparticles in Vibrio -Stimulated Sea Urchin Immune Cells
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Alijagic, Andi (Istituto per la Ricerca e l'Innovazione Biomedica) ;
Bonura, Angela (Istituto per la Ricerca e l'Innovazione Biomedica) ;
Barbero, Francesco (Institut Català de Nanociència i Nanotecnologia) ;
Puntes, Víctor (Institut Català de Nanociència i Nanotecnologia) ;
Gervasi, Francesco (Hospitals Civico Di Cristina e Benfratelli) ;
Pinsino, Annalisa (Istituto per la Ricerca e l'Innovazione Biomedica)
We investigated the role of the gold nanoparticles functionalized with polyvinylpyrrolidone (PVP-AuNPs) on the innate immune response against an acute infection caused by Vibrio anguillarum in an in vitro immunological nonmammalian next-generation model, the sea urchin Paracentrotus lividus. [...]
2021 - 10.3390/nano11102646
Nanomaterials, Vol. 11, Issue 10 (October 2021) , art. 2646
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6.
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32 p, 4.2 MB |
Cross-species comparisons of nanoparticle interactions with innate immune systems : a methodological review
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Swartzwelter, Benjamin (Istituto di Biochimica e Biologia Cellulare (Nàpols, Itàlia)) ;
Mayall, Craig (University of Liubljana. Department of Biology) ;
Alijagic, Andi (Istituto per la Ricerca e l'Innovazione Biomedica (Palerm, Itàlia)) ;
Barbero, Francesco (Institut Català de Nanociència i Nanotecnologia) ;
Ferrari, Eleonora (ZMBP Eberhard-Karls University Tübingen. Center for Plant Molecular Biology) ;
Hernadi, Szabolcs (Cardiff University. School of Biosciences) ;
Michelini, Sara (Paris-Lodron University Salzburg. Department of Biosciences) ;
Navarro Pacheco, Natividad Isabel (Institute of Microbiology of the Czech Academy of Sciences) ;
Prinelli, Alessandra (AvantiCell Science, Ltd.) ;
Swart, Elmer (UK Centre for Ecology and Hydrology (Wallingford, Regne Unit)) ;
Auguste, Manon (Università degli Studi di Genova. Dipartimento di Ambiente terrestre e scienze della vita)
Many components of the innate immune system are evolutionarily conserved and shared across many living organisms, from plants and invertebrates to humans. Therefore, these shared features can allow the comparative study of potentially dangerous substances, such as engineered nanoparticles (NPs). [...]
2021 - 10.3390/nano11061528
Nanomaterials, Vol. 11, issue 6, art. 1528 (June 2021)
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7.
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16 p, 8.0 MB |
Functional and Morphological Changes Induced in Mytilus Hemocytes by Selected Nanoparticles
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Auguste, Manon (University of Genoa. Department of Environmental, Earth, and Life Sciences) ;
Mayall, Craig (Univerza V Ljubljani) ;
Barbero, Francesco (Institut Català de Nanociència i Nanotecnologia) ;
Hočevar, Matej (Institute of Metals and Technology) ;
Alberti, Stefano (University of Genoa. Department of Chemistry and Industrial Chemistry) ;
Grassi, Giacomo (University of Siena. Department of Physical, Earth, and Environmental Sciences) ;
Puntes, Víctor (Institut Català de Nanociència i Nanotecnologia) ;
Drobne, Damjana (Univerza V Ljubljani) ;
Canesi, Laura (University of Genoa. Department of Environmental, Earth, and Life Sciences)
Nanoparticles (NPs) show various properties depending on their composition, size, and surface coating, which shape their interactions with biological systems. In particular, NPs have been shown to interact with immune cells, that represent a sensitive surveillance system of external and internal stimuli. [...]
2021 - 10.3390/nano11020470
Nanomaterials, Vol. 11, Issue 2 (February 2021) , art. 470
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8.
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17 p, 1.5 MB |
Gold Nanoparticles Modulate BCG-Induced Innate Immune Memory in Human Monocytes by Shifting the Memory Response towards Tolerance
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Swartzwelter, Benjamin J. (Institute of Biochemistry and Cell Biology) ;
Barbero, Francesco (Institut Català de Nanociència i Nanotecnologia) ;
Verde, Alessandro (Institute of Biochemistry and Cell Biology) ;
Mangini, Maria (Institute of Biochemistry and Cell Biology) ;
Pirozzi, Marinella (Institute of Biochemistry and Cell Biology) ;
De Luca, Anna Chiara (Institute of Biochemistry and Cell Biology) ;
Puntes, Víctor (Institut Català de Nanociència i Nanotecnologia) ;
Leite, Luciana C. C. (Instituto Butantan. Laboratório de Desenvolvimento de Vacinas) ;
Italiani, Paola (Institute of Biochemistry and Cell Biology) ;
Boraschi, Diana (Institute of Biochemistry and Cell Biology)
Innate immune memory is characterized by a modulation in the magnitude with which innate immune cells such as monocytes and macrophages respond to potential dangers, subsequent to previous exposure to the same or unrelated agents. [...]
2020 - 10.3390/cells9020284
Cells, Vol. 9, Issue 2 (February 2020) , art. 284
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9.
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18 p, 13.4 MB |
Stressor-dependant changes in immune parameters in the terrestrial isopod crustacean, porcellio scaber : a focus on nanomaterials
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Mayall, Craig (Univerza V Ljubljani. Biotehniški fakulteti) ;
Dolar, Andraz (Univerza V Ljubljani. Biotehniški fakulteti) ;
Kokalj, Anita Jemec (Univerza V Ljubljani. Biotehniški fakulteti) ;
Novak, Sara (Univerza V Ljubljani. Biotehniški fakulteti) ;
Razinger, Jaka (Agricultural Institute of Slovenia. Plant Protection Department) ;
Barbero, Francesco (Institut Català de Nanociència i Nanotecnologia) ;
Puntes, Víctor (Institut Català de Nanociència i Nanotecnologia) ;
Drobne, Damjana (Univerza V Ljubljani. Biotehniški fakulteti)
We compared the changes of selected immune parameters of Porcellio scaber to different stressors. The animals were either fed for two weeks with Au nanoparticles (NPs), CeO2 NPs, or Au ions or body-injected with Au NPs, CeO2 NPs, or lipopolysaccharide endotoxin. [...]
2021 - 10.3390/nano11040934
Nanomaterials, Vol. 11 Núm. 4 (April 2021) , art. 934
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10.
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19 p, 7.4 MB |
Gold nanoparticles (AuNPs) impair LPS-driven immune responses by promoting a tolerogenic-like dendritic cell phenotype with altered endosomal structures
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Michelini, Sara (Paris-Lodron University Salzburg) ;
Barbero, Francesco (Institut Català de Nanociència i Nanotecnologia) ;
Prinelli, Alessandra (AvantiCell Science Ltd) ;
Steiner, Philip (University Salzburg) ;
Weiss, Richard (Paris-Lodron University Salzburg) ;
Verwanger, Thomas (Paris-Lodron University Salzburg) ;
Andosch, Ancuela (Paris-Lodron University Salzburg) ;
Lütz-Meindl, Ursula (Paris-Lodron University Salzburg) ;
Puntes, Víctor (Institut Català de Nanociència i Nanotecnologia) ;
Drobne, Damjana (Univerza V Ljubljani) ;
Duschl, Albert (Paris-Lodron University Salzburg) ;
Horejs-Hoeck, Jutta (Paris-Lodron University Salzburg)
Dendritic cells (DCs) shape immune responses by influencing T-cell activation. Thus, they are considered both an interesting model for studying nano-immune interactions and a promising target for nano-based biomedical applications. [...]
2021 - 10.1039/d0nr09153g
Nanoscale, Vol. 13, Issue 16 (April 2021) , p. 7648-7666
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