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| Home > Articles > Published articles > Nanoplastics are bioaccumulated in fish liver and muscle and cause DNA damage after a chronic exposure |
| Home > Articles > Published articles > Nanoplastics are bioaccumulated in fish liver and muscle and cause DNA damage after a chronic exposure |
(Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia) (Universidad de Murcia. Laboratorio Interdisciplinario de Análisis Clínicos Interlab) (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals) (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
| Date: | 2022 |
| Abstract: | The extent of the widespread, planetary contamination by plastic waste is difficult to fully capture. Nanoplastics (NPs) are currently in the center of research concerning plastic litter, both for the analytical challenges they pose and for their potential to provoke hazardous effects in organisms. However, there are still many unanswered questions in this multidisciplinary field, with a crucial missing piece being the quantification of NPs in fish tissues after in vivo exposures. Another relevant question that is still greatly unexplored is how a chronic exposure to NPs will affect fish health. This study aims to provide answers to both of these relevant knowledge gaps. To this end, goldfish (Carassius auratus) were exposed to 44 nm polystyrene (PS)-NPs via water for 30 days. Following the exposure, gastrointestinal tract, liver and muscle were sampled for PS-NPs analysis by means of size exclusion chromatography coupled to high resolution mass spectrometry. PS-NPs were detected in all liver and muscle samples of exposed fish, with higher concentrations in liver than in muscle, whereas no PS-NPs were detected in the gastrointestinal tract. Nevertheless, exposure to PS-NPs did not induce changes in hematology parameters nor in cortisol and glucose levels in plasma. On the other hand, even a relatively low concentration of PS-NPs was able to cause DNA damage, measured by an increase in erythrocyte nuclear abnormalities, suggesting that PS-NPs can reach the cell nucleus and cause genotoxicity. These results show for the first time that PS-NPs find their way to fish muscle after chronic exposure, where they bioaccumulate, but do not alter fish survival nor hematological or physiological stress indicators. The accumulation of PS-NPs in fish muscle can represent a threat to human health as a possible route of exposure to small-sized plastics. The present results in a model fish species open windows for future studies in edible fish species. |
| Grants: | Agencia Estatal de Investigación PID2020-113221RB-I00 Agencia Estatal de Investigación CTM2017-89701-C3-2-R Agencia Estatal de Investigación RYC2019-026841-I Agència de Gestió d'Ajuts Universitaris i de Recerca 2018/FI_B_00711 Ministerio de Ciencia e Innovación PRE2018-083989 Agencia Estatal de Investigación RTI2018-097860-J-I00 |
| Note: | Altres ajuts: acords transformatius de la UAB |
| Rights: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.  |
| Language: | Anglès |
| Document: | Article ; recerca ; Versió publicada |
| Subject: | Nanoplastics ; Quantification ; Chronic ; Genotoxicity ; Liver ; Muscle |
| Published in: | Environmental Research, Vol. 212, Part A (September 2022) , art. 113433, ISSN 1096-0953 |
